| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1251 | Bacillus timonensis | TTCAGTGCCAACGACGGT | GACGGGATGTGACGAATCGT | 59.50 | 60.18 | 258 | 56 |
100.00%
|
100.00%
|
| Primer 1252 | Bacillus timonensis | GGGAAGTTGTTACAGCGCAA | ACCAGACGAACCAACCGT | 59.05 | 58.78 | 201 | 56 |
100.00%
|
100.00%
|
| Primer 1253 | Bacillus timonensis | TTGGGAAGTTGTTACAGCGC | ACCAGACGAACCAACCGT | 59.05 | 58.78 | 203 | 56 |
100.00%
|
100.00%
|
| Primer 1254 | Bacillus timonensis | TCCACTGGACTGCTTGCAC | GATTGCAATTTGCCCAATCGT | 60.23 | 58.38 | 270 | 56 |
100.00%
|
100.00%
|
| Primer 1255 | Bacillus timonensis | TCCACTGGACTGCTTGCA | GATTGCAATTTGCCCAATCGT | 58.76 | 58.38 | 270 | 56 |
100.00%
|
100.00%
|
| Primer 1256 | Bacillus timonensis | TCGGTGCCCTTTTGGTGTT | ACCGCAAATTGGACTCCGT | 60.08 | 59.93 | 268 | 56 |
100.00%
|
100.00%
|
| Primer 1257 | Bacillus timonensis | TTCGGTGCCCTTTTGGTGT | ACCGCAAATTGGACTCCGT | 60.08 | 59.93 | 269 | 56 |
100.00%
|
100.00%
|
| Primer 1258 | Bacillus timonensis | TCGGTGCCCTTTTGGTGT | CCGCAAATTGGACTCCGT | 59.40 | 58.02 | 267 | 56 |
100.00%
|
100.00%
|
| Primer 1259 | Bacillus timonensis | AAGCCATTAGGTGATCGCGT | TCACCAACCGCAACCTCT | 59.82 | 58.76 | 179 | 56 |
100.00%
|
100.00%
|
| Primer 1260 | Bacillus timonensis | GAGGGCTGGATTGAGCGTT | ATGGCTTCCGATGGCTGT | 60.08 | 59.00 | 284 | 55 |
100.00%
|
100.00%
|